The above Tetrad Heatmap will update to represent the tetrad dihedral angles found in the selected conformer with black/white dots. If the selected conformer contains point mutations, the affected tetrads will be highlighted in red and the associated sections of the heatmap will be updated. Once a conformer is selected, mutations can be analyzed below.

Mutations are scored in the context of a seven residue fragment containing the mutation site at the center. If the mutation site does not interact with any residues outside this interaction window (ie: at solvent accessible sites), the prediction should be reliable. Only aqueous conditions near neutral pH are supported. Solvent Accessible Surface Area (SASA) is provided as a percentage to help identify viable mutation sites. Statistics on the number of protein fragments used in the calculation are also included. Multiple fragment datasets are used in each calculation. The "Minimum Samples" statistic reflects the weakest link in the calculation; calculations with less than 20 samples are unreliable. Positive values for ΔΔG are stabilizing.

Multiple output formats are available. In the "Table" format, mutations that are destabilizing by at least 0.2 kcal/mol are colored red and mutations that are stabilizing by at least 0.2 kcal/mol are colored green. The "Heatmap" format shows a grid of all possible mutations colored by stability; The X-axis lists the WT sequence, the Y-axis lists potential mutations. Hovering the mouse over a cell in the grid will reveal the data associated with the mutation. Stabilizing mutations are colored yellow to red, destabilizing mutations are colored cyan to blue. Grey cells do not have data associated with them; dark grey cells are the WT residue, light grey cells were excluded by the calculation parameters. Black cells have insufficient data to perform the calculation.